Skills-Building Programs to Reduce Child Marriage in Bangladesh: A Randomized Controlled Trial.

PURPOSE: Child marriage is the norm in Bangladesh, leading to lifelong negative consequences. Evidence on sustainable child marriage programs is scant. Our study aimed to evaluate the impact of three community-based skills-building programs to delay child marriage among adolescent girls in rural Bangladesh. METHODS: The study used a cluster randomized controlled trial design with four arms-ARM1 offered educational support, ARM2 promoted gender rights awareness, ARM3 offered livelihoods training, and ARM4 was a control area. All adolescent girls were offered 144 hours of skills training in village centers over 18 months. Among 11,609 baseline survey respondents, 91% were successfully included in the endline analysis. Program impact was assessed using discrete time hazard models. RESULTS: The program reduced child marriages (<18) significantly in all arms relative to control-(adjusted hazard ratio [AHR]: .75; 95% confidence interval [CI]: .60-.92) for the education arm, (AHR: .72; 95% CI: .59-.88) for the gender arm, and (AHR: .70; 95% CI: .56-.87) for the livelihoods arm. Program participants were younger and more likely to be in school and faced lower risk of marriage relative to nonparticipants. In the gender and livelihoods arm, nonparticipants had lower risk of child marriage relative to the control group significant at the 10% level. CONCLUSIONS: The study demonstrates it is possible to reduce the prevalence of child marriage in a relatively short period of time by working with communities to implement holistic programs to build skills among girls. The program had similarly large impact and did not depend on the type of skills offered.

Cumulus cell expansion and ultrastructural changes in in vitro matured bovine oocytes under heat stress.

Cumulus-oocyte complexes (COCs) from cows were matured under normal (38.5°C) and elevated temperatures (41°C) simulating heat stress and their maturation was assessed based on measurement of cumulus expansion in both groups. There was a significant reduction (P<0.01) in maturation rate in the heat stressed oocytes. The ultrastructural events associated with in vitro oocyte maturation and changes associated with elevated temperature were also studied by transmission electron microscopy (TEM). Normal maturation cellular events were marked by migration of Golgi and mitochondria from the cortical regions, and conversely by a migration of cortical granules from the inner regions to a sub-perivitelline zone. Heat stressed oocytes (41°C) were not only marked by a reduction in rate and less cumulus cell expansion, but also by a reduction in cortical granule migration. The mitochondria appeared swollen with cristolysis. Ribosomal disruption and an abundance of free ribosomes were also seen. Changes in the cumulus cells include nuclear chromatin margination, condensation and karyolysis, formation of nuclear and cell membrane blebs, and typical membrane bound vesicles enclosing cell fragments indistinguishable from apoptosis. Evidently, heat stress can be associated with reduced cytoplasmic events of oocyte maturation, thereby decreasing the oocyte competence and can be associated with apoptosis of the cumulus cells and therefore compromise the survival of the oocyte itself.

Antimicrobial, Antioxidant, and Cytotoxic Activities of Ocimum forskolei and Teucrium yemense (Lamiaceae) Essential Oils.

Background:Ocimum forskolei and Teucrium yemense (Lamiaceae) are used in traditional medicine in Yemen. Methods: The chemical composition, antimicrobial, antioxidant and cytotoxic activities of the essential oils isolated from the leaves of Ocimum forskolei Benth. (EOOF) and two different populations of Teucrium yemense Deflers., one collected from Dhamar province (EOTY-d), and another collected from Taiz (EOTY-t) were investigated. The antimicrobial activities of the oils were evaluated against several microorganisms with the disc diffusion test or the broth microdilution test. The essential oils were screened for in-vitro cytotoxic activity against human tumor cells. EOOF and EOTY-d were screened for free-radical-inhibitory activity using the DPPH radical scavenging assay. Results: Sixty-four compounds were identified in (EOOF) representing 100% of the oil content with endo-fenchol (31.1%), fenchone (12.2%), τ-cadinol (12.2%), and methyl (E)-cinnamate (5.1%) as the major compounds. In EOTY-d, 67 compounds were identified, which made up 91% of the total oil. The most abundant constituents were (E)-caryophyllene (11.2%), α-humulene (4.0.%), γ-selinene (5.5%), 7-epi-α-selinene (20.1%), and caryophyllene oxide (20.1%), while the major compounds in EOTY-t were α-pinene (6.6%), (E)-caryophyllene (19.1%) α-humulene (6.4%), δ-cadinene (6.5%), caryophyllene oxide (4.3%), α-cadinol (9.5%), and shyobunol (4.6%). The most sensitive microorganisms for EOOF were B. subtilis, S. aureus, and C. albicans with inhibition zones of 34, 16, and 24 mm and MIC values of, 4.3 mg/mL, 4.3 mg/mL, and 8.6 mg/mL, respectively. EOTY-t showed antimicrobial activity against S. aureus, B. cereus, A. niger, and B. cinerea with MIC values of 0.156, 0.156, 0.313 and 0.313 mg/mL, respectively. Neither essential oil showed remarkable radical inhibition (IC50 = 31.55 and 31.41 µL/mL). EOTY-d was active against HT-29 human colorectal adenocarcinoma cell lines with IC50 = 43.7 µg/mL. Consistent with this, EOTY-t was active against both MCF-7 and MDA-MB-231 human breast adenocarcinoma cells. Conclusions: The antimicrobial activity of Ocimum forskolei essential oil against B. subtilis and C. albicans is consistent with its traditional use in Yemeni traditional medicine to treat skin infections. Both O. forskolei and T. yemense show wide variations in their respective essential oil compositions; there remains a need to investigate both species botanically, genetically, and phytochemically more comprehensively.

Variations in Essential Oil Compositions of Lavandula pubescens (Lamiaceae) Aerial Parts Growing Wild in Yemen.

Lavandula pubescens Decne. is one of five Lavandula species growing wild in Yemen. The plant is used in Yemeni traditional medicine, and the essential oil tends to be rich in carvacrol. In this work, L. pubescens was collected from eight different locations in Yemen, the essential oils obtained by hydrodistillation, and the oils analyzed by gas chromatography/mass spectrometry (GC/MS). Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were used to differentiate between the L. pubescens samples. The essential oils were rich in carvacrol (60.9 - 77.5%), with lesser concentrations of carvacrol methyl ether (4.0 - 11.4%), caryophyllene oxide (2.1 - 6.9%), and terpinolene (0.6 - 9.2%). The essential oil compositions in this study showed very high similarity, but it was possible to discern two separate groups based on minor components, in particular the concentrations of terpinolene, carvacrol methyl ether, m-cymen-8-ol, and caryophyllene oxide.

Flooding transition in the topography of toppling surfaces of stochastic and rotational sandpile models.

A continuous phase transition occurs in the topography of toppling surfaces of stochastic and rotational sandpile models when they are flooded with liquid, say water. The toppling surfaces are extracted from the sandpile avalanches that appear due to sudden burst of toppling activity in the steady state of these sandpile models. Though a wide distribution of critical flooding heights exists, a critical point is defined by merging the flooding thresholds of all the toppling surfaces. The criticality of the transition is characterized by power-law distribution of island area in the critical regime. A finite size scaling theory is developed and verified by calculating several new critical exponents. The flooding transition is found to be an interesting phase transition and does not belong to the percolation universality class. The universality class of this transition is found to depend on the degree of self-affinity of the toppling surfaces characterized by the Hurst exponent H and the fractal dimension D(f) of critical spanning islands. The toppling surfaces of different stochastic sandpile models are found to have a single Hurst exponent, whereas those of different rotational sandpile models have another Hurst exponent. As a consequence, the universality class of different sandpile models remains preserved within the same symmetry of the models.

Parasitological aspects of Capillaria philippinensis recovered from Egyptian patients.

Seven new cases of intestinal capillariasis were detected in Kasr El-Aini University Hospitals, Cairo, during the period from July 1997 to June 1998. A parasitological study concerning the morphological features of Capillaria philippinensis recovered from the stool of Egyptian cases was carried out. Eggs were separated, cultured and used to infect Oreochromis nilotica niloticus, a famous popularly consumed type of fresh water fish in Egypt in a trial for identification of the fish intermediate host for this parasite, but infection had not developed in the intestine of this species of fish.